Vitiligo is a multifactorial disease characterized by skin depigmentation. Although there are several genetic components involved in its development, the participation of the TYR rs7129973 gene polymorphism has not yet been explored. Our objective was to evaluate the association between vitiligo vulgaris and the TYR rs7129973 gene polymorphism in a Mexican population. Therefore, a total of 84 vitiligo vulgaris patients and 90 control subjects from northeastern Mexico were analyzed through PCR-RFLP to determine the association between vitiligo and TYR rs7129973. We found that the carriers of TYR rs7129973 G alleles (AG and GG genotypes) were more prevalent among patients with vitiligo (P<0.05). However, this genetic variant had no correlation with the age of onset, vitiligo activity, family history of vitiligo, and personal history of thyroid disease (P>0.05). Thereby, we can conclude that the TYR rs7129973 polymorphism constitutes a risk factor for the development of vitiligo vulgaris in the Mexican population.